Product Description
4 x 96 Preps
Zymo D4053″ title=”ZR-96 DNA Sequencing Clean-up Kit,
4 x 96 Preps
Zymo D4053″ />ZR-96 DNA Sequencing Clean-up Kit,
4 x 96 Preps
Zymo D4053
Storage Temperature: Room Temperature
The ZR-96 DNA Sequencing Clean-up Kit provides a simple method for the rapid removal of post-cycle sequencing reaction contaminants (i.e., unincorporated fluorescent dyes, residual salts, dNTPs, primers, and enzymes) from DNA extension products. These contaminants can often interfere with the quality and signal strength of sequencing data. In particular, unincorporated dyes can result in dye peaks (“dye blobs”) which may obscure portions of the sequencing chromatogram and interfere with base-calling accuracy of sequencing analysis software. DNA is eluted with a small volume of water or loading dye containing formamide. The entire DNA purification procedure typically takes about 10 minutes.
Complete elimination of “Dye Blobs” for high quality Phred scores and long read lengths.Flexible 15-20 µl elution volumes allow for direct loading of samples with no precipitation or drying steps.Reusable!
Elution Volume: ≥ 15 µl of DNA Elution BufferEquipment: Centrifuge with microplate carriersSample Source: Sequencing input samples with post-cycle sequencing reaction contaminants from DNA extension productsSample storage: Eluted DNA can be used immediately or stored at ≤ -20°CYield: Up to 5 µg DNA
